miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cells

Abstract

ObjectiveTo explore the biological role of miR-206 in inhibiting the proliferation and invasion of estrogen-induced ER-36 positive gastric cancer cells and its related mechanisms. MethodsMTT and tranwell assays were used to detect the proliferation and invasion of BGC-823 cells under different concentrations and time of ER stimulation. RT-PCR was used to detect the effect of ER stimulation on the content of miR-206 in gastric cancer cells. miR-206 mimics were transfected into BGC-823 cells to detect cell proliferation and invasion; luciferase reporter assay was used to determine whether miR-206 targets the 3-untranslated region of the CDK14-encoding gene (3 -UTR). ResultsWe confirmed that estrogen promoted the proliferation and invasion of BGC-823 cells; As the concentration of estrogen increases, the level of miR206 in the cell gradually rises.Overexpression of miR-206 significantly decreased the estrogen-induced BGC-823 cells. Proliferative and invasive ability; miR-206 inhibits the expression of CDK14 by directly binding to the 3-UTR of CDK14 mRNA, thereby inhibiting the proliferation and invasion of gastric cancer cells. ConclusionsOur results suggest that miR-206 inhibits estrogen-induced proliferation and invasion of ER-36-positive gastric cancer cells by inhibiting CDK14 and may be a promising therapeutic option for anticancer therapy.