Science AdvancesMolecular BiologyPeer-reviewed journal article
CRISPR-based engineering of RNA viruses
Artem A. NemudryiiD, Anna NemudraiaiD, Joseph E. NicholsiD, Andrew M. ScherffiusiD, Trevor ZahliD, Blake WiedenheftiD
Abstract
CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a new recombinant RNA technology with immediate applications for the facile engineering of RNA viruses. One-Sentence SummaryProgrammable CRISPR RNA-guided ribonucleases enable recombinant RNA technology.
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- 8
- FWCI
- 0.71
- Field percentile
- top 36%
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