O. R. Burrone

Recruitment of TRiC chaperonin in rotavirus viroplasms directly associates with virus replication.

This study identifies TRiC chaperonin as a component of rotavirus viroplasms essential for dsRNA synthesis and virus replication.

Rotavirus spike protein VP4 mediates viroplasm assembly by association to actin filaments

This study shows that rotavirus VP4 interacts with actin filaments to facilitate viroplasm assembly, using a recombinant virus with a modified VP4 to demonstrate the role of this interaction.

A recombinant rotavirus harboring a spike protein with a heterologous peptide reveals a novel role of VP4 in viroplasm stability

This paper engineers a recombinant rotavirus with a VP4-BAP tag, showing VP4's role in viroplasm stability and replication fitness.

Rotavirus Replication Factories Are Complex Ribonucleoprotein Condensates

This study shows rotavirus replication factories are RNA-protein condensates formed by phase separation, with unique features that could be targeted for antiviral therapies.

CRISPR-Csy4-mediated editing of rotavirus double-stranded RNA genome

This study uses CRISPR-Csy4 to edit rotavirus dsRNA genome, enabling labeling of secondary transcription products and insights into viral replication.

Recombinant rotaviruses rescued by reverse genetics reveal the role of NSP5 hyperphosphorylation in the assembly of viral factories

This study uses reverse genetics to generate recombinant rotaviruses with NSP5 mutations, revealing that NSP5 hyperphosphorylation is essential for viroplasm assembly and viral replication.

The guanine nucleotide exchange factor GBF1 participates in rotavirus replication

This study shows that GBF1 is required for rotavirus replication by facilitating VP7 and NSP4 processing and virion assembly.