GENETHOFF: a flexible workflow for genome wide profiling of CRISPR/Cas off targets
Guillaume CorreiD, Marine Rouillon, Margaux MomblediD, Amendola MarioiD
Abstract
We developed GENETHOFF, a flexible single-command versatile Snakemake workflow designed for the comprehensive analysis of CRISPR/Cas9 related OFF-targets genomic positions from GUIDE-Seq derived protocols. It efficiently processes multiplexed libraries from different organisms, PCR orientations, and Cas nucleases with varying PAM specificities in a single run, all based on a simple user-specified datasheet containing sample metadata.
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