Rongjie Fu

Acquired resistance to the PRMT5 inhibitor confers collateral sensitivity to MEK inhibition in MTAP-null non-small cell lung cancer

The paper identifies collateral sensitivity to MEK inhibition in MTAP-null NSCLC cells that develop resistance to PRMT5 inhibitors.

Mechanistic Insights into Proteomic Mutation-Phenotype Linkages from Tiling Mutagenesis Screens

The paper introduces ProTiler-Mut, a framework for analyzing tiling mutagenesis screens to uncover mutation-phenotype linkages, revealing new insights into functional mutation categories and protein interactions.

Guide-specific loss of efficiency and off-target reduction with Cas9 variants

The paper evaluates efficiency and off-target effects of high-fidelity Cas9 variants with different sgRNAs and develops a computational framework to predict their performance.

Systematic decomposition of sequence determinants governing CRISPR/Cas9 specificity

This study systematically identifies sequence determinants of CRISPR/Cas9 specificity by analyzing off-target effects of 1,902 gRNAs on 13,314 targets, revealing three key factors and developing a predictive tool.

De novo Identification of Essential Protein Domains from CRISPR/Cas9 Tiling-sgRNA Knockout Screens

The paper introduces ProTiler, a method to identify essential protein domains from CRISPR/Cas9 tiling-sgRNA screens, finding 175 CKHS regions with high overlap to annotated domains and novel unannotated domains.