Wei He

The paper identifies collateral sensitivity to MEK inhibition in MTAP-null NSCLC cells that develop resistance to PRMT5 inhibitors.

The paper introduces ProTiler-Mut, a framework for analyzing tiling mutagenesis screens to uncover mutation-phenotype linkages, revealing new insights into functional mutation categories and protein interactions.

The paper introduces JAGWAS, a multi-phenotype GWAS tool that identifies more genetic loci for brain imaging phenotypes than single-phenotype GWAS.

The paper evaluates efficiency and off-target effects of high-fidelity Cas9 variants with different sgRNAs and develops a computational framework to predict their performance.

The paper defines 481 conserved 3D ligand-binding motifs based on functional groups using an alignment-free method, applicable to drug-target interactions.

This study systematically identifies sequence determinants of CRISPR/Cas9 specificity by analyzing off-target effects of 1,902 gRNAs on 13,314 targets, revealing three key factors and developing a predictive tool.

GuidePro integrates multiple predictive methods to prioritize sgRNAs for CRISPR/Cas9 protein knockouts, showing consistent performance across datasets.

The paper introduces ProTiler, a method to identify essential protein domains from CRISPR/Cas9 tiling-sgRNA screens, finding 175 CKHS regions with high overlap to annotated domains and novel unannotated domains.

EASY-RNAseq is a low-input RNAseq protocol that improves gene detection sensitivity and expression distribution in small cell populations.