AePUb promoter length modulates gene expression in Aedes aegypti
Michelle A. E. AndersoniD, Philip T. LeftwichiD, Ray Wilson, Leonela Carabajal PaladinoiD, Sanjay BasuiD, Sara RooneyiD, Zach N. AdelmaniD, Luke AlpheyiD
Abstract
Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.
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