Philip T. Leftwich

The paper investigates fitness differences between knock-in and knock-out cd mutants in Anopheles stephensi, finding that knock-in mutants have reduced survival and midgut dysfunction, while knock-outs do not.

The paper identifies and validates a 7SK RNA polymerase III promoter in Ceratitis capitata for use in CRISPR/Cas9 gene editing strategies.

This study shows that sequence mismatches between gene-drive and target-site flanking regions reduce homing efficiency in Culex quinquefasciatus by up to 54%.

Across nearly one million progeny from 10 species, species best predicts homing drive inheritance, while promoter, parent sex and maternal deposition each explain little of the inheritance variation.

The paper assesses two multiplexing strategies for CRISPR/Cas9-based gene drives in Anopheles stephensi to overcome target site resistance.

This paper demonstrates the feasibility of using CRISPR-Cas9 gene drives in the medfly to achieve sex conversion and female sterility, offering a strategy for pest population suppression.

The paper shows that varying the length of the AePUb promoter in Aedes aegypti can adjust reporter gene expression levels in both cell culture and mosquitoes.

The paper develops a synthetic biology approach to modulate gene expression in mosquitoes by systematically characterizing modifications in translation initiation sequences and 3' UTRs.

This study demonstrates CRISPR-based gene drives in Culex quinquefasciatus, showing biased inheritance of transgenes with modest efficiency.

This study tests a CRISPR/Cas9 split-drive system in the diamondback moth, showing high somatic editing but limited germline activity.

This paper shows that expressing the scorpion toxin AaHIT in diamondback moth reduces adult lifespan and female egg production, demonstrating a non-cell-autonomous genetic pest management approach.