Luke Alphey

The paper develops and validates an SDA-500 Anopheles stephensi cell line for molecular studies, showing it supports efficient transgene expression and functional genomics research.

The paper investigates fitness differences between knock-in and knock-out cd mutants in Anopheles stephensi, finding that knock-in mutants have reduced survival and midgut dysfunction, while knock-outs do not.

This study shows that sequence mismatches between gene-drive and target-site flanking regions reduce homing efficiency in Culex quinquefasciatus by up to 54%.

This study quantifies LSDV transmission by three insect vectors, finding Stomoxys calcitrans as the most efficient with R0 of 5.8, while Culicoides nubeculosus did not transmit the virus.

The paper assesses two multiplexing strategies for CRISPR/Cas9-based gene drives in Anopheles stephensi to overcome target site resistance.

The paper shows that varying the length of the AePUb promoter in Aedes aegypti can adjust reporter gene expression levels in both cell culture and mosquitoes.

The paper develops a synthetic biology approach to modulate gene expression in mosquitoes by systematically characterizing modifications in translation initiation sequences and 3' UTRs.

This study demonstrates CRISPR-based gene drives in Culex quinquefasciatus, showing biased inheritance of transgenes with modest efficiency.

This study investigates inheritance bias mechanisms in a CRISPR gene drive in Aedes aegypti, finding two distinct mechanisms: homing in double heterozygous males and meiotic drive.

The paper identifies small silencing RNAs targeting PxyMasc, a male-determining gene in diamondback moth, expressed from W-linked retrocopies on the female-specific chromosome.

This study tests a CRISPR/Cas9 split-drive system in the diamondback moth, showing high somatic editing but limited germline activity.

This paper shows that expressing the scorpion toxin AaHIT in diamondback moth reduces adult lifespan and female egg production, demonstrating a non-cell-autonomous genetic pest management approach.

This paper models mutation and reversal of engineered underdominance gene drives, showing mutations can break them and free suppressors are less effective than wild-type releases for reversal.