A sensitive and specific genetically encodable biosensor for potassium ions
S.-Y. Wu, Y. Wen, N. B. C. Serre, C. C. H. Laursen, A. G. Dietz, B. R. Taylor, A. Aggarwal, V. Rancic, M. Becker, K. Ballanyi, K. Podgorski, H. Hirase, M. Nedergaard, M. Fendrych, M. J. Lemieux, D. F. Eberl, A. R. Kay, R. E. Campbell, Y. Shen
Abstract
Potassium ions (K+) play a critical role as an essential electrolyte in all biological systems. Here we report the crystal structure-guided optimization and directed evolution of an improved genetically encoded fluorescent K+ biosensor, GINKO2. GINKO2 is highly sensitive and specific for K+ and enables in vivo detection of K+ dynamics in multiple species.
02 /Specification & Classification
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