E. S. Boyden

Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections

The paper introduces a method called patch-walking to improve the efficiency of finding synaptic connections by reusing a single patch clamp electrode, resulting in more connections probed per experiment.

HiExM: high-throughput expansion microscopy enables scalable super-resolution imaging

HiExM enables scalable super-resolution imaging by expanding cells in 96-well plates, improving drug effect detection in cardiomyocytes.

Visualizing proteins by expansion microscopy

This paper introduces ONE microscopy, a technique that enables nanoscale imaging of single proteins and molecular complexes, revealing structural details in cells and disease samples.

A Multifunctional Anchor for Multimodal Expansion Microscopy

This paper introduces a multifunctional anchor for expansion microscopy, enabling simultaneous imaging of multiple biomolecules with reduced cost and complexity.

Decrowding Expansion Pathology: Unmasking Previously Invisible Nanostructures and Cells in Intact Human Brain Pathology Specimens

The paper introduces dExPath, an expansion microscopy variant that improves immunostaining in human brain pathology specimens by expanding proteins and reducing autofluorescence, revealing previously invisible disease markers.

Tuning the sensitivity of genetically encoded fluorescent potassium indicators through structure-guided and genome mining strategies

The paper develops genetically encoded fluorescent potassium indicators with tunable binding affinities through structure-guided design and genome mining, showing varying Kd values in vitro and in cells.

Expansion Revealing: Decrowding Proteins to Unmask Invisible Brain Nanostructures

Expansion Revealing decrowds proteins to reveal previously invisible nanostructures in brain tissue, including presynaptic calcium channel alignment and amyloid nanoclusters in Alzheimer's models.

Post-transcriptional splicing can occur in a slow-moving zone around the gene

The paper uses imaging techniques to show that splicing occurs in a slow-moving zone near the transcription site, suggesting a model where splicing is post-transcriptional but occurs close to transcription.

Expansion Microscopy of Lipid Membranes

This paper introduces membrane expansion microscopy (mExM), a technique for nanoscale optical imaging of lipid membranes with improved resolution for studying protein-lipid interactions in organelles.

A highly homogeneous expansion microscopy polymer composed of tetrahedron-like monomers

This paper introduces a new expansion microscopy polymer with a more homogeneous network structure, achieving higher resolution and reduced spatial errors in imaging.

Highly multiplexed in situ protein imaging with signal amplification by Immuno-SABER

This paper introduces Immuno-SABER, a method for highly multiplexed protein imaging that overcomes limitations in sensitivity and throughput.

A theoretical analysis of single molecule protein sequencing via weak binding spectra

This paper proposes a theoretical method for single molecule peptide sequencing using weak binding probes, showing it can achieve high accuracy with low-affinity binders.

Imaging beyond the super-resolution limits using ultrastructure expansion microscopy (UltraExM)

This paper introduces UltraExM, a method that enables subdiffraction-resolution imaging of preserved ultrastructures using standard optical microscopy, revealing new insights into centriolar structures.

Rapid Sequential In Situ Multiplexing With DNA-Exchange-Imaging

This paper introduces DNA-Exchange-Imaging, a method for rapid, highly multiplexed in situ protein visualization, achieving sub-20 nm resolution and reducing imaging time from days to minutes.