henhaobio
Sign in

bioRxivCell BiologyPreprint

Microtubule Probe for Correlative Super-Resolution Fluorescence and Electron Microscopy

X. T. Battaglia, C. De Pace, L. Ruiz-Perez, B. Chen, R. Su, M. Zhang, R. Zhang, Q. Zhang, Q. Wang, H. Zhou, J. Wu, G. Battaglia, Z. Zhang, Y. Tian

PDFDOI

Abstract

We report a versatile cyclometalated Iridium (III) complex probe that achieves synchronous fluorescence-electron microscopy correlation to reveal microtubule ultrastructure in cells. The selective insertion of probe between repeated and {beta} units of microtubule triggers remarkable fluorescent enhancement, and high TEM contrast due to the presence of heavy Ir ions. The highly photostable probe allows live cell imaging of tubulin localization and motion during cell division with an resolution of 20 nm, and under TEM imaging reveals the {beta} unit interspace of 45[A] of microtubule in cells.

Reproducibility ledger

Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.

Sign in to file a reproducibility report.

Related papers

Similarity Map
SelectedSame fieldCross-field
THISGerasimaite '21Spector '24Paul '19Simmert '18Auvray '25Gwosch '19
68% semantic matchCell Biology
R. Gerasimaite, J. Bucevicius, K. A. Kiszka+3 more·bioRxiv·Jun 1, 2021