bioRxivBiochemistryPreprint
A cell type-resolved proteomic atlas of the human body
Caroline A M WeissiD, Evelina SjöstedtiD, Adhiraj Debnath, Shani Ben‐MosheiD, Lucas DiedrichiD, Martin ZwahleniD, Marc OelleriD, Maria WahleiD, Emilio Skarwan, Tim HeymanniD, Denys OliinykiD, Elena KratziD, Andreas MetousisiD, Christian Braun, Heribert SchunkertiD, Fredric JohanssoniD, Emma K. LundbergiD, Kalle von FeilitzeniD, Cecilia LindskogiD, Florian A. RosenbergeriD, Moritz von ScheidtiD, Mathias UhléniD, Matthias ManniD
- Citations
- 2
Abstract
Proteins define what cells do, yet their systematic quantification across human cell types has remained out of reach. Using Deep Visual Proteomics on tissue from a healthy female donor, we built an atlas of 27 cell types across 14 tissues, quantifying two-thirds of all human protein-coding genes, with up to 8,500 per population. The proteome partitions bimodally into a universal core and highly specialized programs. Integration into the Human Protein Atlas Single Cell Resource enabled comparison of RNA and protein abundance at cell type resolution, revealing that concordance depends on pathway rather than cellular identity. This resolution uncovered cancer-testis antigens in oocytes invisible to bulk profiling. Our openly accessible resource provides a foundation for cell type-resolved proteomics in health and disease.
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