Influence of rifampicin-resistance mutations on the synthesis of antioxidant, DNA-protective, and SOS-inhibitory metabolites

Abstract

This work is aimed at studying the effects of rifampicin resistance mutations on the synthesis of secondary metabolites with antioxidant and DNA-protective properties. We used probiotic strains of the genus Bacillus: B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933. The antioxidant, DNA-protective activity, and the ability to suppress the SOS-response in B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 rifampicin-resistant mutants have been studied for the first time. It has been found that antioxidant, DNA protective, and SOS-inhibiting activity is higher in rifampicin-resistant mutants than that of original strains. According to the study results, it has been discovered that the antioxidant, DNA-protective and SOS-inhibitory activity in mutants B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 resistant to rifampicin is higher than in control strains, which indirectly proves the pleiotropic effect of the rpoB gene on these activities.