K. Y. Rhee

The Bacillus subtilis yqgC-sodA operon protects magnesium-dependent enzymes by supporting manganese efflux

This paper shows that deletion of the yqgC-sodA operon in Bacillus subtilis causes Mn sensitivity by reducing Mn efflux and inhibiting Mg-dependent enzymes, distinct from efflux-deficient mutants.

Mutations in rpoB that confer rifampicin resistance can alter levels of peptidoglycan precursors and affect β-lactam susceptibility

This paper studies how rpoB mutations in Bacillus subtilis confer rifampicin resistance and affect β-lactam susceptibility through changes in peptidoglycan precursor levels.

Predicting enzyme substrate chemical structure with protein language models

This paper predicts chemical substrate structures for orphan enzymes using protein language models, achieving high accuracy for Mycobacterium tuberculosis SDRs and SAM-methyltransferases.

Genome-wide co-essentiality analysis in Mycobacterium tuberculosis reveals an itaconate defense enzyme module

The paper uses co-essentiality analysis of TnSeq data to identify gene modules in Mtb, focusing on an itaconate defense enzyme cluster and proposing novel functional associations.

Listeria monocytogenes GlmR is an accessory uridyltransferase essential for cytosolic survival and virulence

This study identifies GlmR as an accessory uridyltransferase essential for Listeria monocytogenes cytosolic survival and virulence by synthesizing UDP-GlcNAc.

Multiple acyl-CoA dehydrogenase deficiency kills Mycobacterium tuberculosis in vitro and during infection

This study identifies EtfDMtb as an essential dehydrogenase for fatty acid β-oxidation in Mtb, showing that disrupting it kills the bacterium in vitro and in mice.

Chemical-genetic interaction mapping links carbon metabolism and cell wall structure to tuberculosis drug efficacy

This study maps chemical-genetic interactions in Mycobacterium tuberculosis to identify how carbon metabolism and cell wall structure affect drug efficacy, revealing environment-dependent and -independent interactions.

The Mycobacterium tuberculosis transposon sequencing database (MtbTnDB): a large-scale guide to genetic conditional essentiality

The paper builds MtbTnDB, a database of Mtb TnSeq screens, enabling comparison of genetic essentiality across conditions and analysis of gene function.

Inhibiting Mycobacterium tuberculosis CoaBC by targeting a new allosteric site.

The paper identifies potent inhibitors of Mycobacterium tuberculosis CoaB by targeting a novel allosteric site, based on structural and biochemical analyses of CoaBC.