The WPRE Improves Genetic Engineering With Site-Specific Nucleases
Jessica M. Ong, Christopher R. BrowniD, Matthew C. MendeliD, Gregory J. CostiD
Abstract
AbstractInclusion of the woodchuck hepatitis virus post-transcriptional response element (WPRE) in the 3 UTR of mRNA encoding zinc-finger or TALE nucleases results in up to a fifty-fold increase in nuclease expression and a several-fold increase in nuclease-modified chromosomes. Significantly, this increase is additive with the enhancement generated by transient hypothermic shock. The WPRE-mediated improvement is seen across several types of human and mouse primary and transformed cells and is translatable in vivo to the mouse liver.
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