A universal method for gene expression engineering
R. Lale, L. Tietze, J. Nesje, I. Onsager, K. Engelhardt, C. F. A. Wong, M. Akan, N. Hummel, J. Kalinowski, C. Rueckert, M. F. Hohmann-Marriott
Abstract
The precise expression of genes is one of the foundations of biotechnology. Here we present GeneEE, a straightforward method for generating artificial gene expression systems. We demonstrate that GeneEE segments, containing a 200 nucleotide DNA with random nucleotide composition, can facilitate constitutive and inducible gene expression. To highlight the universal character of our method, we demonstrate GeneEE-mediated gene and protein expression in six bacterial species and Bakers yeast.
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