bioRxivNeurosciencePreprint
A FRET-Based FLIM Method to Probe Membrane-Induced Alpha-Synuclein Aggregation in Neurons
Paula-Marie E. IveyiD, Abdelrahman M. SalemiD, Sehong MiniD, Wenzhu Qi, Magaly Guzman Sosa, Tamara L. Kinzer‐UrsemiD, Jean‐Christophe RochetiD, Kevin John WebbiD
Abstract
1Parkinsons disease (PD) involves the aggregation of the protein alpha-synuclein, a process promoted by interactions with intracellular membranes. To study this phenomenon in neurons for the first time, we developed a fluorescence lifetime imaging (FLIM) method using Forster resonance energy transfer and self-quenching reporters, analyzed with a custom-built FLIM microscope. This method offers insights into aggregate formation in PD and can be broadly applied to probe protein-membrane interactions in neurons.
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