P. S. McPherson

DENND6A couples Arl8b to a Rab34/RILP/dynein complex regulating retrograde lysosomal trafficking and autophagy

This study identifies DENND6A as a Rab34 GEF that couples with Arl8b to regulate lysosomal retrograde trafficking and autophagy.

DENND2B activates Rab35 at the intercellular bridge regulating cytokinetic abscission and tetraploidy

This study identifies DENND2B as a Rab35 GEF that regulates cytokinetic abscission and prevents tetraploidy by modulating actin dynamics and the NoCut checkpoint.

The identification of potent and selective antibodies for Serine/threonine-protein kinase TBK1, for use in immunoblot, immunofluorescence and immunoprecipitation

The paper evaluates 11 commercial antibodies for TBK1 detection in immunoblot, immunofluorescence, and immunoprecipitation using a knock-out cell line to identify potent and selective antibodies.

Rapid macropinocytic transfer of α-synuclein to lysosomes

This study shows alpha-synuclein fibrils are rapidly internalized via macropinocytosis and directed to lysosomes, with some transferred to neighboring cells.

Host kinase CSNK2 is a target for inhibition of pathogenic β-coronaviruses including SARS-CoV-2

The paper shows that inhibiting the kinase CSNK2 blocks replication of several beta-coronaviruses, including SARS-CoV-2, by preventing viral entry.

Role of the ubiquitin ligase ITCH in clathrin-mediated endocytosis of the epidermal growth factor receptor

This study shows that ITCH is required for clathrin-mediated endocytosis of EGFR and regulates its trafficking and signaling.

SARS-CoV-2 infects cells following viral entry via clathrin-mediated endocytosis

This study shows SARS-CoV-2 enters cells via clathrin-mediated endocytosis, and inhibiting clathrin reduces viral infectivity.

An Arf/Rab cascade controls the growth and invasiveness of glioblastoma

This study shows that loss of Rab35 or Arf5 in glioblastoma cells increases tumor growth and invasiveness by activating the epidermal growth factor receptor and upregulating SPOCD1.