Jean‐Christophe Rochet

Autism-associated SCN2A deficiency disrupts cortico-striatal circuitry in human brain assembloids

This study shows that SCN2A deficiency in human brain assembloids disrupts cortico-striatal circuitry by impairing axonal projections and synaptic transmission, leading to hyperexcitability.

Patient-derived tau-seeded human neuronal chimeras recapitulate mature Alzheimer's tau pathology and uncover human-specific neuronal vulnerability

This study develops a human neuronal chimera model to study Alzheimer's tau pathology, showing human-specific vulnerability and recapitulating mature AD features in vivo.

FLIM-FRET as a Molecular Filter for Membrane-Induced Aggregation

This paper introduces a FLIM-FRET method to quantify membrane-proximal alpha-synuclein aggregation in neurons by estimating per-cell lifetimes and mixture fractions using a hierarchical EM algorithm.

Tau-seed interactome analysis reveals distinct functional signatures in Alzheimer's disease across model systems.

The paper identifies distinct protein interactors of Tau-seed in AD across species and brain regions, highlighting functional differences in Tau propagation.

Human microglia in brain assembloids display region-specific diversity and respond to hyperexcitable neurons carrying SCN2A mutation

This study uses brain organoids and assembloids with microglia to show region-specific microglial diversity and their response to SCN2A mutation-induced neuronal hyperactivity.

Mechanisms of Alpha-Synuclein Seeded Aggregation in Neurons Revealed by Fluorescence Lifetime Imaging

This study uses fluorescence lifetime imaging to track alpha-synuclein aggregation in neurons, revealing that seeds disassemble before escaping endolysosomes and triggering cytosolic aggregation.

Alzheimer's Disease Patient Brain Extracts Induce Multiple Pathologies in Vascularized Neuroimmune Organoids for Disease Modeling and Drug Discovery

This study uses vascularized neuroimmune organoids to model sporadic Alzheimer's disease and test drug efficacy.

A FRET-Based FLIM Method to Probe Membrane-Induced Alpha-Synuclein Aggregation in Neurons

The paper develops a FLIM method to study membrane-induced alpha-synuclein aggregation in neurons, providing insights into Parkinson's disease mechanisms.

Human iPSC-derived microglia sense and dampen hyperexcitability of cortical neurons carrying the epilepsy-associated SCN2A-L1342P mutation

This study shows human iPSC-derived microglia detect and reduce hyperexcitability in neurons with the SCN2A-L1342P epilepsy mutation through morphological and calcium changes and reduced sodium channel current density.

Effects of Neighboring Phosphorylation Events on the Affinities of pT181-Tau Antibodies

The paper investigates how phosphorylation near the pT181 epitope affects the affinity of pT181-tau antibodies, finding that phosphorylation at S184 or S185 can completely eliminate antibody binding.

Protective effects of polyphenol-rich extracts against neurotoxicity elicited by paraquat or rotenone in cellular models of Parkinson's disease

The paper investigates the neuroprotective effects of polyphenol-rich extracts against paraquat and rotenone-induced neurotoxicity in Parkinson's disease models, identifying mechanisms involving antioxidant pathways and mitochondrial function.

Monitoring phagocytic uptake of amyloid β into glial cell lysosomes in real time

This study develops a fluorescent Aβ analogue to monitor glial cell phagocytosis of amyloid β in real time, showing microglia uptake exceeds astrocytes.

Novel Small Molecules Targeting the Intrinsically Disordered Structural Ensemble of α-Synuclein Protect Against Diverse α-Synuclein Mediated Dysfunctions

This study identifies small molecules that target the intrinsically disordered ensemble of α-synuclein, showing they can inhibit misfolding, restore phagocytosis, and block cellular transmission of α-synuclein.

Phospholipid membranes promote the early stage assembly of α-synuclein aggregates

This study shows phospholipid bilayers catalyze α-synuclein aggregation from monomers, with efficiency depending on membrane composition and leading to dissociable aggregates.