Unmasking Epstein-Barr Role in Gastric Carcinogenesis: A Gene Expression Approach to Virus-Positive Tumors

Abstract

Human gammaherpesvirus 4 or Epstein-Barr virus (EBV) is an oncogenic virus linked to malignancies like gastric adenocarcinoma. Notably, EBV infection induces genetic and epigenetic modifications that play a crucial role in oncogenesis and tumor progression, underscoring the importance of analyzing viral gene expression in the context of gastric cancer (GC) to elucidate its unique characteristics. This study aimed to perform a molecular characterization of EBV gene expression using next-generation sequencing (NGS). The analysis included human gene expression patterns in EBV-positive and EBV-negative samples and the expression of viral genes in EBV-positive samples. The study received approval from the Ethics and Research Committee of Joao de Barros Barreto University Hospital under reference number 47580121.9.0000.5634. It utilized 76 tumor tissue samples from patients with gastric cancer who had undergone surgical resection, and both fresh and paraffin-embedded samples were gathered for total RNA sequencing (RNA-seq) and in situ hybridization (ISH). The RNA-seq was conducted in a pair-end manner on the NextSeq(R) platform (Illumina(R), US). The NextSeq(R) 500 MID Output V2 kit - 150 cycles (Illumina(R)) were utilized following the manufacturers instructions. ISH targeting RNA-1 of EBER1 (Y5200, DAKO, Carpinteria) was performed using the automated Dako system. Molecular characterization was conducted using the Kraken2 software. Subsequently, to elucidate the mechanisms through which EBV may influence gastric cancer, we analyzed the patterns of human gene expression in EBV-positive and EBV-negative samples. Of the 76 samples, 8 were classified as EBV-positive according to the applied methodology. Our analysis identified approximately 834 differentially expressed genes, 92 of which exhibited an AUC > 0.85. These genes are implicated in tumor progression, cellular metabolism, and both innate and adaptive immune responses. Additionally, viral genes expressed in the positive samples were evaluated, and we found manifestations of both lytic phase and latent phase genes. Finally, our study presents an efficient strategy for molecular classification of EBV-positive gastric cancer based on NGS and shows the effects of EBV on human gene expression. Author summaryIn our study, we explored how EBV influences the development of stomach cancer. EBV is a virus known to be linked to various cancers, including gastric cancer, and it can alter the behavior of both human and viral genes within infected cells. To investigate this, we analyzed tissue samples from 76 patients with stomach cancer, focusing on differences between samples with and without EBV. Using advanced sequencing technology, we identified over 800 genes that behave differently in EBV-positive cancers. These genes are involved in critical processes like how cells grow, how the immune system responds, and how energy is produced within cells. We also examined which EBV genes were active in the cancer samples and found evidence of both dormant and active phases of the virus. Our work demonstrated how EBV may contribute to stomach cancer and suggests new ways to classify and understand this disease. By uncovering these details, we hope to pave the way for more targeted treatments in the future.