A nematic framework for sprouting angiogenesis

Abstract

Sprouting angiogenesis is critical for embryogenesis, wound healing, and tumor growth. Here we show that the liquid crystal framework, which has recently been leveraged to study various morphogenetic events, provides unique insight into angiogenic sprouting. In response to vascular endothelial growth factor, a potent pro-angiogenic factor, endothelial cells cultured on the surfaces of soft collagen hydrogels form elongated cell streams that coexist with more polygonal cell regions. Angiogenic sprouting initiates preferentially at cell stream tips, where nematic order gradients are most pronounced. We demonstrate that cell streams are associated with large traction forces that lead to 3D deformations of the underlying hydrogel and that stream formation results from a delicate balance between microtubule polymerization and actomyosin contractility. Finally, we show that active control of nematic order allows modulation of sprout formation, highlighting the key role of EC orientation gradients in angiogenic sprouting and paving the way for new therapeutic avenues. TeaserA nematic perspective reveals that sprouting angiogenesis correlates with elevated gradients in endothelial cell orientation.