Plant biochemistry and biosynthesis

This study identifies three cytochrome P450 monooxygenase cyclases responsible for the final steps of glyceollin biosynthesis in soybean.

This study overexpresses SmERF6 in Salvia officinalis to enhance the production of diterpenes like carnosol and carnosic acid, showing significant increases in their levels.

The paper identifies Moraceae-specific P450 enzymes involved in furanocoumarin biosynthesis by analyzing evolutionary patterns and functional divergence in Ficus carica.

The paper reviews putative reaction pathways for the biosynthesis of sesquiterpene coumarins derived from 7-farnesyloxycoumarins, highlighting their evolutionary link to triterpene biosynthesis.

This paper demonstrates the production and functionalization of heterologous sesquiterpenoids in the plastid of Chlamydomonas reinhardtii using STPs and soluble CYPs, achieving up to 80% functionalization and 250-2500 µg L-1 yields.

This study identifies and characterizes three new CYPs in T. litoralis that catalyze specific oxidations of four MIA skeletons, expanding understanding of MIA biosynthesis.

The paper identifies cassava accessions with enhanced β-carotene content and examines its relationship with starch, lipid, and protein biosynthesis pathways.

This study models how circadian rhythms affect terpenoid precursor biosynthesis in plants, showing that combined regulation maintains stable concentrations despite daylight variations.

The paper identifies the biosynthetic origin of the methoxy group in quinine and related Cinchona alkaloids, showing it is introduced onto tryptamine and demonstrating the pathway in planta.

The paper constructs a cell-type-aware gene regulatory network for MIA biosynthesis in C. roseus using single-cell multi-omics data, identifying a novel idioblast-specific TF that regulates late-stage biosynthetic genes.

This study identifies two phylogenetically distant enzymes, CfADH1 and CfAKR2b, localized in cytosol and plastids, that drive citral biosynthesis in lemongrass.

This paper identifies and characterizes pachysiphine synthase genes in Tabernaemontana elegans using genome sequencing and computational analysis, enabling engineered yeast production of MIAs.

The paper solves the crystal structure of caryolan-1-ol synthase with and without a substrate analog, revealing structural changes that suggest a mechanism for anti-Markovnikov cyclization.

This study shows that substrate channeling in PaFS occurs without a flexible linker or covalent domain linkage, and that cyclase domains associate with the prenyltransferase octamer for optimal channeling.

The paper identifies two CYP450 enzymes in Tabernanthe iboga responsible for oxidative modifications of tabersonine scaffolds using a streamlined screening strategy in Nicotiana benthamiana.

This paper identifies and functionally characterizes CYP76BK1 orthologs across Lamiaceae subfamilies, showing their role in furanoclerodane biosynthesis and evolutionary conservation.

This study identifies and characterizes a new gammaTMT-like enzyme from Tabernaemontana elegans, showing parallel evolution of methyltransferases leading to perivine N-methylation in two plant species.

The paper develops Triterpene RDF, a manually curated database of plant enzymes and transcription factors involved in triterpene biosynthesis, to improve functional annotation of genomes and transcriptomes.

This study examines how pipecolic acid treatment affects terpenoid composition in different tissues of tansy plants, revealing tissue-specific responses and metabolic differentiation.

The paper identifies Hyper cells in St. John's wort where hyperforin biosynthesis occurs de novo and characterizes four prenyltransferases involved in its synthesis.

Three CYP716 enzymes in Nicotiana attenuata catalyze distinct steps in triterpene biosynthesis, influencing growth and development through ABA and stress responses.

The paper identifies one-step anthranilate methyltransferases in grapes that synthesize methyl anthranilate, revealing distinct active site residues compared to maize and citrus enzymes.

The paper identifies biosynthetic genes and pathways for phenylphenalenones and linear diarylheptanoid derivatives in Musella lasiocarpa using transcriptomics and biochemical assays.

This study evaluates Nepeta cataria as a host for transient expression and metabolic engineering of secondary biosynthetic pathways, showing lower lichenase activity compared to Nicotiana benthamiana.

This study identifies and characterizes RcDT1, a prenyltransferase involved in anthraquinone biosynthesis in Rubia cordifolia, and shows its evolutionary origin from the ubiquinone pathway.

This study identifies CrROP3 and CrROP5 as geranylgeranylated ROP GTPases that positively regulate monoterpene indole alkaloid biosynthesis in Catharanthus roseus.

This study evaluates color, scent, and their interaction in corona and perianth tissues of Iranian narcissus genotypes, identifying VOCs, carotenoids, and gene expression differences.

This paper reconstitutes an early paclitaxel biosynthetic network by engineering Taxus genes to increase taxadien-5-ol accumulation and identify pathway byproducts.

This study investigates the evolution and functional diversification of carboxylesterase-like cyclases in aspidosperma and iboga alkaloid biosynthesis using phylogenetic and biochemical approaches.

The paper identifies and functionally characterizes a root-specific terpene synthase in Artemisia pallens that produces major root volatiles.