Construction and characterization of a genome-scale ordered mutant collection of Bacteroides thetaiotaomicron
H. A. Arjes, J. Sun, H. Liu, T. H. Nguyen, R. N. Culver, A. I. Celis, S. J. Walton, K. S. Vasquez, F. B. Yu, K. S. Xue, D. P. Newton, R. Zermeno, M. Weglarz, A. Deutschbauer, K. Huang, A. L. Shiver
The paper constructs an ordered mutant collection of Bacteroides thetaiotaomicron using transposon-insertion and barcode sequencing, identifies factors limiting coverage, and demonstrates phenotypic screening for growth defects and cell shape changes.
bioRxiv · Dec 27, 2021Microbiology