W. Chiu

Proliferative cell targeting and epithelial cell turnover fuels hepatitis E virus replication in human intestinal enteroids

This study establishes an HEV-HIE model showing HEV replication in intestinal epithelial cells, with proliferative cells being the main targets, suggesting the gut as an HEV reservoir.

Dramatic Changes in Mitochondrial Subcellular Location and Morphology Accompany Activation of the CO2 Concentrating Mechanism

This study shows that mitochondrial repositioning and reorientation in Chlamydomonas reinhardtii under low CO2 conditions is regulated by CIA5 and supports CO2 concentrating mechanism function.

Cost-benefit analysis of cryogenic electron tomography subtomogram averaging of chaperonin MmCpn at near atomic resolution.

This study analyzes the factors affecting resolution in cryoET-STA for MmCpn, evaluating the cost-benefit of achieving high-resolution structures.

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

This paper determines the 3D structures of SL5 RNA domains from six coronaviruses, revealing conserved tertiary features with implications for protein-binding and antiviral targets.

Structure of the Thin Filament in Human iPSC-derived Cardiomyocytes and its Response to Heart Disease

This study uses cryo-ET to analyze the structure of thin filaments in human iPSC-derived cardiomyocytes and how they change with disease-related perturbations.

CryoEM structures of the human CLC-2 voltage gated chloride channel reveal a ball and chain gating mechanism

The paper reports cryoEM structures of human CLC-2 chloride channels, revealing a ball-and-chain gating mechanism involving an N-terminal hairpin peptide.

Single-dose YF17D-vectored Ebola vaccine candidate protects mice against both lethal surrogate Ebola and yellow fever virus challenge

This study develops a dual-target vaccine against Ebola and yellow fever viruses, showing it protects mice against both pathogens with a single dose.

Caught in the Act: targeted mutagenesis of the herpesvirus fusogen central helix captures transition states

This study mutates the herpesvirus fusogen gB to capture its transition state, revealing the critical role of domain III in membrane fusion.

Cryogenic electron tomography reveals novel structures in the apical complex of Plasmodium falciparum

This study uses cryogenic electron tomography to reveal new structural details of the apical complex in Plasmodium falciparum merozoites, including interactions between rhoptries and other structures.

Coupling cryo-electron tomography with mixed-scale dense neural networks reveals re-organization of the invasion machinery of Toxoplasma gondii upon ionophore-stimulation

The paper uses cryo-electron tomography and mixed-scale dense neural networks to analyze the reorganization of Toxoplasma gondii's invasion machinery upon ionophore stimulation, revealing structural changes and evolutionary conservation with Plasmodium falciparum.

3D RNA-scaffolded wireframe origami

This paper designs, fabricates, and characterizes 3D RNA-scaffolded wireframe origami structures using different RNA sequences, revealing structural insights and demonstrating the potential for functional RNA scaffolds.

Development and optimization of biologically contained Marburg virus for high-throughput antiviral screening

This paper develops a biologically contained Marburg virus system for high-throughput antiviral screening and validates its safety and utility.

Molecular Architecture of the Chikungunya Virus Replication Complex

This study determines the structure of the chikungunya virus replication complex at near-atomic and subnanometer resolution, revealing its molecular architecture and implications for antiviral therapy.

Structural visualization of the tubulin folding pathway directed by eukaryotic chaperonin TRiC

The paper visualizes the tubulin folding pathway mediated by TRiC using cryoEM, showing how TRiC's structure guides the folding of β-tubulin through specific interactions.

Development and optimisation of a high-throughput screening assay for in vitro anti-SARS-CoV-2 activity: evaluation of 5676 phase 1 passed structures

The paper develops a high-throughput screening assay to evaluate 5676 phase 1 compounds for in vitro anti-SARS-CoV-2 activity, identifying eight candidates but finding only remdesivir suitable for further clinical development.

Remdesivir, Molnupiravir and Nirmatrelvir remain active against SARS-CoV-2 Omicron and other variants of concern.

The paper evaluates the antiviral activity of remdesivir, molnupiravir, and nirmatrelvir against SARS-CoV-2 variants, finding they remain effective.

Identification of novel Ebola virus inhibitors using biologically contained virus

This study screens over 4,200 small-molecule compounds against biologically contained Ebola virus to identify novel inhibitors.

Previously uncharacterized rectangular bacterial structures in the dolphin mouth

The paper discovers novel rectangular bacterial structures in dolphin mouths and characterizes their morphology and potential bacterial origin through multiple methods.

In situ Alphavirus Assembly and Budding Mechanism Revealed by Cellular CryoET

This study uses cryoET to reveal the in situ assembly and budding mechanism of CHIKV, showing spike-driven particle formation and NAb inhibition of spike-spike interactions.

Discovery of pan-ErbB inhibitors protecting from SARS-CoV-2 replication, inflammation, and lung injury by a drug repurposing screen

This study identifies lapatinib, an ErbB inhibitor, as a broad-spectrum antiviral that suppresses SARS-CoV-2 and VEEV replication, inflammation, and lung injury through ErbB-mediated mechanisms.

Near-atomic resolution Cryo-EM structure of Mayaro virus identifies key structural determinants of alphavirus particle formation

The paper presents a near-atomic resolution cryo-EM structure of Mayaro virus, revealing structural determinants of alphavirus particle formation and identifying key residues involved in assembly.

The N-terminus of varicella-zoster virus glycoprotein B has a functional role in fusion.

This study identifies the N-terminus of VZV glycoprotein B as critical for membrane fusion, showing that mutations in this region impair viral propagation.

A single immunization with spike-functionalized ferritin vaccines elicits neutralizing antibody responses against SARS-CoV-2 in mice

This study shows that a single dose of spike-functionalized ferritin vaccines in mice generates neutralizing antibodies against SARS-CoV-2, with S{Delta}C-Fer showing higher efficacy than other spike formats.

Resolving Individual-Atom of Protein Complex using Commonly Available 300-kV Cryo-electron Microscopes

The paper demonstrates near-atomic resolution structure determination of apoferritin using single-particle cryo-EM with a 300-kV Titan Krios microscope, achieving 1.35 Å resolution and resolving heavy atoms and rotameric configurations.

A 3.4-A cryo-EM structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles

The paper presents a 3.4-A cryo-EM structure of the human coronavirus NL63 spike trimer from vitrified virus particles, revealing structural differences from SARS-CoV-2.

Cryo-electron Microscopy and Exploratory Antisense Targeting of the 28-kDa Frameshift Stimulation Element from the SARS-CoV-2 RNA Genome

The paper uses cryo-EM and ASO experiments to study the SARS-CoV-2 FSE structure and its potential as an antiviral target, finding a complex tertiary structure but challenges in effective inactivation.

Multi-Scale 3D Cryo-Correlative Microscopy for Vitrified Cells

This paper introduces a multi-scale 3D cryo-correlative microscopy pipeline for visualizing vitrified cells, demonstrating its application to heat-shocked yeast cells.

STAT2 signaling as double-edged sword restricting viral dissemination but driving severe pneumonia in SARS-CoV-2 infected hamsters

This study shows that STAT2 signaling both restricts SARS-CoV-2 spread and causes severe pneumonia in infected hamsters.

Crystal and cryo-EM structures of the cytosolic G protein alpha chaperone and guanine nucleotide exchange factor Ric-8A bound to Gαi1

This study reports the crystal and cryo-EM structures of Ric-8A bound to Gαi1, revealing its mechanism of GTP exchange and chaperone activity through structural interactions.

Evidence for influenza B virus hemagglutinin adaptation to the human host: high cleavability, acid-stability and preference for cool temperature

This study compares influenza B virus (IBV) and influenza A virus (IAV) hemagglutinin (HA) adaptation to human airways, finding IBV HA is more cleavable, acid-stable, and temperature-preferred for human upper airways.