The paper develops MATAC-Seq to analyze single-molecule chromatin accessibility at replication origins, identifying chromatin features that predict origin efficiency in yeast.

bioRxiv · Mar 14, 2023Biochemistry

Myeloid-biased HSC require Semaphorin 4A from the bone marrow niche for self-renewal under stress and life-long persistence

D. Toghani, S. Zeng, E. Mahammadov, E. I. Crosse, N. Seyedhassantehrani, C. Burns, D. Gravano, S. Radtke, H.-P. Kiem, S. Rodriguez, N. Carlesso, A. Pradeep, N. K. Wilson, S. J. Kinston, B. Gottgens, , L. Silberstein

This study identifies Sema4A as a critical niche factor required for myeloid-biased HSC self-renewal and long-term persistence under stress.

bioRxiv · Jan 12, 2022 · PreprintCell Biology

The role of cell geometry and cell-cell communication in gradient sensing

J. Fiorentino, A. Scialdone

This study generalizes mathematical models of gradient sensing in epithelial tissues to analyze how cell number, geometry, and communication range affect gradient measurement in 2D systems.

bioRxiv · Oct 13, 2021Biophysics

Epstein-Barr virus reprograms human B-lymphocytes immediately in the pre-latent phase of infection

P. Mrozek-Gorska, A. Buschle, D. Pich, T. Schwarzmayr, R. Fechtner, A. Scialdone, W. Hammerschmidt

This study shows EBV rapidly reprograms primary human B-lymphocytes transcriptionally within 24 hours of infection, leading to metabolic changes and cell cycle entry by day three.

bioRxiv · Dec 31, 2018Microbiology