Molecular BiologyCommunications BiologyPeer-reviewed
Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template
Eric J. Aird, Klaus N. Lovendahl, Amber St. Martin…·Wendy Ryan Gordon
This paper enhances HDR efficiency by covalently tethering a donor DNA template to the Cas9/guide RNA complex, achieving up to 8-fold improvement in several editing assays.
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