Klaus N. Lovendahl

Reconstructing a Missing Link of HIV-1 Assembly: HIV-1 Envelope-Matrix Interactions in a Native Viral Context

This study investigates HIV-1 Env-MA interactions using cryo-ET and simulations, revealing the Kennedy-sequence motif's role in Env incorporation and Gag maturation's effect on Env clustering.

Structure of HIV-1 Env glycoprotein on virions reveals an alternative fusion subunit organization and native membrane coupling

This study reveals conformational differences in HIV-1 Env glycoprotein on virions compared to soluble forms, highlighting the role of the viral membrane in maintaining native structure.

Interprotomer Crosstalk in Mosaic Viral Glycoprotein Trimers Provides Insight into Polyvalent Immunogen Co-assembly

This study investigates the structural dynamics of mosaic SARS-CoV-2 spike trimers formed by co-assembling different variants, showing preserved stability and antigenicity with potential implications for polyvalent vaccine design.

Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

This study develops a method to efficiently edit multiple genes in human T cells using base editors, reducing off-target effects and improving safety for immunotherapy.

Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template

This paper enhances HDR efficiency by covalently tethering a donor DNA template to the Cas9/guide RNA complex, achieving up to 8-fold improvement in several editing assays.