Reuben S. Harris

A SARS-CoV-2 entry inhibitor trimerizes to lock the spike protein in a closed conformation

This paper describes a trimeric inhibitor, S22, that stabilizes the SARS-CoV-2 spike protein in a closed conformation, preventing ACE2 binding and viral entry.

Scarless SARS-CoV-2 Genome Engineering and Variant Analysis

This paper develops a scarless genome engineering system for SARS-CoV-2 to study protease inhibitors and drug resistance mechanisms, showing improved antiviral potency when combined with drug efflux inhibitors.

Genetic studies with a uracil DNA glycosylase biosensor support a role for mitochondrial UNG1 in nuclear uracil repair

The paper develops a biosensor to measure UNG activity in living cells and shows mitochondrial UNG1 contributes to nuclear uracil repair.

APOBEC3G Splicing Defects in Nonhuman Primate Models Result in Disparate Viral Mutational Profiles Relative to Humans

This study identifies APOBEC3G splicing defects in nonhuman primates that lead to different viral mutation profiles compared to humans.

Endogenous APOBEC3B Promotes CHK1 Inhibitor Sensitivity

This study shows that endogenous APOBEC3B causes DNA damage and replication stress, making cancer cells sensitive to CHK1 inhibitors.

Collapsing retroviruses for efficient delivery of viro-toxic cargoes

This paper introduces collapsing retroviruses (CRVs) to deliver viro-toxic cargoes like APOBEC3B by using recombination to restore gene integrity during transduction, achieving near 100% efficiency.

Full-length structure of the anti-viral and pro-tumor DNA deaminase APOBEC3B

This paper reports the cryoEM structure of full-length APOBEC3B in complex with BORF2, revealing its domain organization and mechanism of viral neutralization and mutagenesis.

Murine models for triple-negative breast cancer with differential responsiveness to immunotherapy

The paper develops two murine TNBC models with differing immunotherapy responses for studying treatment mechanisms and therapies.

HAMMER: Hairpin-based APOBEC3A-mediated mRNA editing reporter

HAMMER is a luminescence-based assay that measures APOBEC3A-mediated RNA editing by detecting changes in luciferase activity ratios.

Mus Musculus papillomavirus MmuPV1 resists restriction by human APOBEC3B

This study shows human APOBEC3B does not restrict murine papillomavirus MmuPV1 replication in cellulo or in vivo, suggesting papillomaviruses have evolved mechanisms to evade this antiviral factor.

Mammalian ZAP and KHNYN can independently restrict CpG-enriched avian viruses

The paper shows mammalian ZAP and KHNYN can restrict CpG-enriched avian viruses, with human ZAP or KHNYN independently limiting CpG-enriched IAV in chicken and human cells.

Structural basis for sequence context-independent single-stranded DNA cytosine deamination by the bacterial toxin SsdA

This study reports the crystal structure of SsdA, a bacterial ssDNA deaminase, revealing its unique substrate binding mechanism and sequence-independent activity.

APOBEC3 mutagenesis drives therapy resistance in breast cancer

This study identifies APOBEC3 mutagenesis as a common driver of therapy resistance in breast cancer, associated with poorer clinical outcomes.

Structural Insights into the Assembly and Regulation of 2'-O RNA Methylation by SARS-CoV-2 nsp16/nsp10

This study presents a 2.4 Å crystal structure of the SARS-CoV-2 nsp16/nsp10 complex bound to Cap-1 RNA, revealing structural insights into its assembly and methylation mechanism.

Regulatory interactions between APOBEC3B N- and C-terminal domains

This study models the full-length APOBEC3B structure and finds that NTD-CTD interactions increase active site opening frequency.

Faecalibacterium prausnitzii regulates carbohydrate metabolic functions of the gut microbiome in C57BL/6 mice

This study shows that the F. prausnitzii strain UT1 alters gut microbiome composition and carbohydrate metabolism in C57BL/6 mice.

Carcinogen-induced DNA adducts amplify APOBEC mutagenesis through nucleotide excision repair

This study shows that carcinogen-induced DNA adducts enhance APOBEC3B-mediated mutagenesis, leading to increased mutation rates in cancer genomes.

A real-time biochemical assay for quantitative analyses of APOBEC-catalyzed DNA deamination

The paper develops a real-time biochemical assay to quantify APOBEC3-catalyzed DNA deamination and identify inhibitors.

Computational Discovery of an Allosteric Pocket on APOBEC3B and the Impact of Metal Contamination on Inhibitor Validation

This study identifies a novel allosteric pocket on APOBEC3B using computational methods but finds that metal contamination in screening samples led to false positive inhibitor results.

APOBEC shapes tumor evolution and age at onset of lung cancer in smokers

The paper identifies two lung cancer subtypes defined by APOBEC mutagenesis levels and shows their association with different genetic mutations, aging, and immune responses.

Acute expression of human APOBEC3B in mice causes lethality associated with RNA editing

This study shows that overexpressing human APOBEC3B in mice causes lethality through RNA editing and organ dysfunction.

Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template

This paper enhances HDR efficiency by covalently tethering a donor DNA template to the Cas9/guide RNA complex, achieving up to 8-fold improvement in several editing assays.