Wendy Ryan Gordon

This paper introduces programmable antibody-DNA conjugation methods using HUH-tags to enable quantitative measurement of receptor-specific adhesion dynamics in cancer cells and NK cells.

This paper demonstrates the use of a non-cytotoxic aerolysin mutant to non-genetically functionalize cell surfaces with proteins, enzymes, and DNA.

This study engineers HUH-tags to form sequence-directed covalent bonds with RNA, enhancing their reactivity through directed evolution.

This paper develops a DNA-based tension sensor platform to measure mechanical forces in cells, enabling direct quantification of drug-induced changes in force transmission for mechanotherapeutic discovery.

This study develops a Notch inhibitory nanobody and demonstrates a strategy to enhance its efficacy through fusion with an Aerolysin domain.

HUHgle is a tool for designing and optimizing HUH-tag mediated protein-ssDNA conjugates, predicting formation efficiency and specificity.

This paper describes a split Staphylococcus aureus prime editor delivered by dual AAVs, demonstrating in vitro editing capacity despite lower-than-ideal efficiency.

This study develops a method to efficiently edit multiple genes in human T cells using base editors, reducing off-target effects and improving safety for immunotherapy.

The paper introduces SNAPS, a toolkit to study proteolytic switches in transmembrane receptors, identifies new switches, and demonstrates its use in screening and therapeutic testing.

This paper enhances HDR efficiency by covalently tethering a donor DNA template to the Cas9/guide RNA complex, achieving up to 8-fold improvement in several editing assays.