Disulfide constrained Fabs overcome target size limitation for high-resolution single-particle cryo-EM
Jennifer E. Kung, Matthew Camp Johnson, Dimitry Tegunov, Christine C. Jao, Ping Wu, Angela Oh, May H. Lin, Jose M. Daria, Christopher M. Koth, Christopher P. Arthur, Alexis L. Rohou, Jawahar Sudhamsu
This paper introduces Rigid-Fabs, disulfide-constrained antibody fragments that enable high-resolution cryo-EM structures of small proteins by overcoming size limitations.
Nature Communications · Sep 30, 2025Peer-reviewedBiophysics