Machine learning guided cell-free expression maps the biochemical landscape of carbonic anhydrase
John Tyler Lazar, Evan Komp, Irene Martinez, Kyle Zolkin, Pascal Notin, Samer Saleh, Grant M. Landwehr, K I Kim, Anru Tian, Benjamin Shapero, Ashty S. Karim, Debora S. Marks, Gregg T. Beckham, Michael C. Jewett
The paper uses machine learning to guide cell-free expression of carbonic anhydrases, identifying variants with improved stability under high temperatures.
bioRxiv · Jul 8, 2026 · PreprintSynthetic Biology