Carbon-conserving Bioproduction of Malate in an E. coli-based Cell-Free System
Ryan A. L. Cardiff, Shaafique Chowdhury, Widianti Sugianto, Benjamin I. Tickman, Diego Alba Burbano, Pimphan A. Meyer, Margaret Cook, Brianne King, David Garenne, Alexander S. Beliaev, Vincent Noireaux, Pamela Peralta‐Yahya, James M. Carothers
This paper engineers a carbon-conserving cell-free system in E. coli to produce malate from formate and bicarbonate, achieving a 15-fold increase in titer and reducing background reactions by 6-fold.
Metabolic Engineering · Apr 8, 2025Peer-reviewedSynthetic Biology