Synthetic BiologyMetabolic EngineeringPeer-reviewed
Carbon-conserving Bioproduction of Malate in an E. coli-based Cell-Free System
Ryan A. L. Cardiff, Shaafique Chowdhury, Widianti Sugianto…·James M. Carothers
This paper engineers a carbon-conserving cell-free system in E. coli to produce malate from formate and bicarbonate, achieving a 15-fold increase in titer and reducing background reactions by 6-fold.
7 citations