Diego Alba Burbano

This paper introduces cSAGE, a conjugation-based genome engineering platform that enables site-specific integration in transformation-resistant bacteria, demonstrating its utility in bioproduction pathway prototyping.

This paper engineers a carbon-conserving cell-free system in E. coli to produce malate from formate and bicarbonate, achieving a 15-fold increase in titer and reducing background reactions by 6-fold.