A Targeted Genome-scale Overexpression Platform for Proteobacteria
Amy B. Banta, Ashley N. Hall, Kevin S. Myers, Ryan D. Ward, Bryce C. Davis, Rodrigo A. Cuellar, Michael Place, Claire C. Freeh, Emily E. Bacon, Jason M. Peters
The paper develops CRISPRtOE, a genome-scale overexpression platform for Proteobacteria, demonstrating its use in Escherichia coli and Zymomonas mobilis to identify antibiotic and engineering targets.
bioRxiv · Mar 1, 2024 · PreprintSystems Biology