Ryan David Ward

This study uses metagenomics to analyze cyanosphere microbiomes associated with terrestrial cyanobacteria, revealing host-specific and environmentally influenced microbial communities and functional differences.

The paper identifies surface receptor masking and internal defenses as barriers to phage therapy in Pseudomonas aeruginosa clinical isolates and presents strategies to overcome these barriers through phage engineering.

This study uses CRISPR interference to screen chemical inhibitors against essential genes in A. baumannii, revealing gene-inhibitor interactions and potential drug targets.

This study identifies Pseudomonas aeruginosa genes whose partial knockdown increases vulnerability in a murine pneumonia model, highlighting potential targets for antibacterial strategies.

The paper develops CRISPRtOE, a genome-scale overexpression platform for Proteobacteria, demonstrating its use in Escherichia coli and Zymomonas mobilis to identify antibiotic and engineering targets.

This study identifies genetic vulnerabilities and antibiotic sensitivities in Acinetobacter baumannii using CRISPRi, revealing key targets and interactions for therapeutic development.

This study identifies conditionally essential genes in Zymomonas mobilis for aerobic and anaerobic growth using CRISPRi-seq, revealing roles of DNA repair and respiratory complexes in energy coupling and isoprenoid biosynthesis.