C. A. Castaneda

Phase separation of polyubiquitinated proteins in UBQLN2 condensates controls substrate fate

This study shows that polyubiquitinated substrates modulate UBQLN2 phase separation in a linkage-dependent manner, affecting proteasome activity and deubiquitination.

Short N-terminal disordered regions and the proline-rich domain are major regulators of phase transitions for full-length UBQLN1, UBQLN2 and UBQLN4

This study shows that the N-terminal disordered regions and proline-rich domain regulate phase separation in UBQLN1, UBQLN2, and UBQLN4, with distinct temperature dependencies.

Decoding optimal ligand design for multicomponent condensates

This study identifies optimal polyubiquitin ligand architectures that promote UBQLN2 phase separation by analyzing the energetic penalties and spatial configurations of Ub units.

Ion mobility mass spectrometry unveils global protein conformations in response to conditions that promote and reverse liquid-liquid phase separation

This study uses ion mobility mass spectrometry to analyze how protein conformations change during and reverse liquid-liquid phase separation.

Mechanistic insights into the enhancement or inhibition of phase separation by polyubiquitin chains of different lengths or linkages

This study investigates how different polyubiquitin chain lengths and linkages affect UBQLN2's liquid-liquid phase separation, showing that extended chains enhance while compact chains inhibit it.

Previously uncharacterized interactions between the folded and intrinsically disordered domains impart asymmetric effects on UBQLN2 phase separation

This study identifies previously uncharacterized interactions between folded and disordered domains of UBQLN2 that asymmetrically modulate its phase separation behavior.

ALS-linked mutations impair UBQLN2 stress-induced biomolecular condensate assembly in cells

This study investigates how ALS-linked mutations in UBQLN2 affect stress-induced biomolecular condensate formation and properties in cells.