C. Chanez

Molecular mechanism of plasmid elimination by the DdmDE defense system

This study uses cryo-EM to elucidate the mechanism of plasmid elimination by the DdmDE defense system in Vibrio cholerae.

An Alpha-helical Lid Guides the Target DNA toward Catalysis in CRISPR-Cas12a

This study reveals how the alpha-helical lid in CRISPR-Cas12a guides the DNA target strand toward the RuvC catalytic core through molecular dynamics simulations and experiments.

Structural basis for RNA-mediated assembly of type V CRISPR-associated transposons

This study presents a cryo-EM structure of a CRISPR-associated transposon complex, revealing RNA-mediated assembly mechanisms and the role of ribosomal protein S15 in transposition.

Mutations in DNA polymerase δ subunit 1 mediate CMD2-type resistance to Cassava Mosaic Geminiviruses

The paper identifies a nonsynonymous SNP in MePOLD1 as the causal mutation for CMD2-type resistance to Cassava Mosaic Geminiviruses, validated through virus-induced gene silencing and allele analysis.

Molecular mechanism of target site selection and remodeling by type V CRISPR-associated transposons

This study elucidates the molecular mechanism of RNA-directed DNA transposition by type V-K CRISPR-associated transposons using cryo-EM structures and biochemical assays.