L. Loeff

DNA end sensing and cleavage by the Shedu anti-phage defense system

This study characterizes the Shedu anti-phage defense system, revealing its DNA end recognition and cleavage mechanism, and shows how phages evade it by altering DNA replication.

Molecular mechanism of plasmid elimination by the DdmDE defense system

This study uses cryo-EM to elucidate the mechanism of plasmid elimination by the DdmDE defense system in Vibrio cholerae.

Mechanistic basis for oxidative stress protection of the human tRNA ligase complex by the oxidoreductase PYROXD1

The paper identifies how PYROXD1 protects the tRNA ligase complex from oxidative stress through structural and functional interactions.

Mechanism of R-loop formation and conformational activation of Cas9

This study reports cryo-EM structures of Cas9 during R-loop formation, revealing conformational changes that activate the nuclease domain and improve understanding of its specificity.

Selective Prespacer Processing Ensures Precise CRISPR-Cas Adaptation

This study identifies how Cas1-Cas2 selects and processes prespacers for CRISPR-Cas adaptation using single-molecule fluorescence and DnaQ exonucleases.

Repetitive DNA reeling by the Cascade-Cas3 complex in nucleotide unwinding steps

This study investigates the DNA unwinding mechanism of the Cascade-Cas3 complex using single-molecule FRET, revealing a spring-loaded reeling process that compensates for inefficient nicking.