CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary cells
M. G. Kluesner, W. S. Lahr, C.-L. Lonetree, B. A. Smeester, P. N. Claudio-Vazquez, S. P. Pitzen, M. J. Vignes, S. C. Lee, S. P. Bingea, A. A. Andrew, B. R. Webber, B. S. Moriarity
The paper compares CBE and ABE base editors for splice-site disruption in T-cells, finding CBEs more reliable and splice-donor targeting most effective.
bioRxiv · Apr 16, 2020Bioengineering