Author

W. S. Lahr

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 5 Immunology· 2 Cancer Biology· 1

Signature Assays, Organisms & Methods

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Organism:human· 7 Approach:genetic perturbation / CRISPR· 6 Research Focus:cell type annotation· 5 Research Focus:drug response / drug discovery· 5 Approach:experimental assay· 4 Research Focus:gene regulatory network inference· 4 Assay:CRISPR screen· 3 Research Focus:cancer & tumor microenvironment· 1

Frequent Co-Authors

B. R. Webber· 8 shared B. S. Moriarity· 6 shared E. J. Pomeroy· 3 shared J. G. Skeate· 3 shared N. J. Slipek· 3 shared B. Moriarity· 2 shared J. B. Krueger· 2 shared M. Choudhry· 2 shared

Activity by Year

2018-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 31 mutual co-author links

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8 shared papersBioengineering

B. R. Webber

Mutual co-authors in cluster

Author profile →

8 papers

Recent·Most cited

Precision Enhancement of CAR-NK Cells through Non-Viral Engineering and Highly Multiplexed Base Editing

M. Wang, J. B. Krueger, A. K. Gilkey, E. M. Stelljes, M. G. Kluesner, E. J. Pomeroy, J. G. Skeate, N. J. Slipek, W. S. Lahr, P. N. Claudio Vazquez, Y. Zhao, E. J. Eaton, K. Laoharawee, B. R. Webber, B. S. Moriarity

This study enhances CAR-NK cell functionality using non-viral base editing and transposon integration, showing improved therapeutic potential in Burkitt's lymphoma models.

bioRxiv · Mar 5, 2024Bioengineering

Homology mediated end joining enables efficient non-viral targeted integration of large DNA templates in primary human T cells

B. R. Webber, M. J. Johnson, N. S. Slipek, W. S. Lahr, A. P. DeFeo, J. G. Skeate, X. Qiu, B. Rathmann, M. D. Diers, B. Wick, T. Henley, M. Choudhry, R. S. McIvor, B. S. Moriarity

This paper describes a non-viral method for efficiently integrating large DNA into primary human T cells using CRISPR-Cas9 and homology-mediated end joining, achieving high functionality and anti-tumor activity.

bioRxiv · Nov 12, 2021 · PreprintBioengineering

Non-Viral Engineering of CAR-NK and CAR-T cells using the Tc Buster Transposon System™

E. J. Pomeroy, W. S. Lahr, J. W. Chang, J. B. Krueger, B. J. Wick, N. J. Slipek, J. G. Skeate, B. R. Webber, B. S. Moriarity

This paper demonstrates a non-viral method using the Tc Buster transposon system to deliver CAR constructs to primary human NK and T cells, achieving efficient gene expression and functional activity.

bioRxiv · Aug 2, 2021 · PreprintBioengineering

Internal checkpoint regulates T cell neoantigen reactivity and susceptibility to PD1 blockade

D. C. Palmer, B. R. Webber, Y. Patel, M. J. Johnson, C. M. Kariya, W. S. Lahr, M. R. Parkhurst, J. J. Gartner, T. D. Prickett, F. J. Lowery, R. J. Kishton, D. Gurusamy, Z. Franco, S. K. Vodnala, M. D. Diers, , N. P. Restifo

This study shows that CISH knockout in TIL enhances neoantigen reactivity and synergizes with PD1 blockade to improve tumor regression.

bioRxiv · Sep 24, 2020Immunology

Engineering T cells to enhance 3D migration through structurally and mechanically complex tumor microenvironments

E. D. Tabdanov, N. J. Rodriguez-Merced, A. Cartagena-Rivera, V. Puram, M. Callaway, E. Ensminger, E. Pomeroy, K. Yamamoto, W. S. Lahr, B. R. Webber, B. Moriarity, A. Zhovmer, P. Provenzano

This study engineers T cells to improve 3D migration through tumor microenvironments by manipulating microtubule stability and contractility, showing enhanced migration in 3D matrices and tumors.

bioRxiv · Apr 21, 2020Cancer Biology

CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary cells

M. G. Kluesner, W. S. Lahr, C.-L. Lonetree, B. A. Smeester, P. N. Claudio-Vazquez, S. P. Pitzen, M. J. Vignes, S. C. Lee, S. P. Bingea, A. A. Andrew, B. R. Webber, B. S. Moriarity

The paper compares CBE and ABE base editors for splice-site disruption in T-cells, finding CBEs more reliable and splice-donor targeting most effective.

bioRxiv · Apr 16, 2020Bioengineering

A Genetically Engineered Primary Human Natural Killer Cell Platform for Cancer Immunotherapy

E. J. Pomeroy, J. T. Hunzeker, M. T. Kluesner, M. R. Crosby, W. S. Lahr, L. Bendzick, J. S. Miller, B. R. Webber, M. A. Geller, B. Walcheck, M. Felices, T. K. Starr, B. S. Moriarity

This paper develops a CRISPR/Cas9-based method to edit human NK cells, enhancing their anti-cancer activity by knocking out ADAM17 and PD1.

bioRxiv · Sep 29, 2018Immunology

Highly Multiplexed Genome Engineering Using CRISPR/Cas9 gRNA Arrays

M. Kurata, N. K. Wolf, W. S. Lahr, M. T. Weg, S. Lee, K. Hui, M. Shiraiwa, B. R. Webber, B. Moriarity

This paper develops a CRISPR/Cas9 gRNA array system to enable efficient multiplex genome engineering by delivering up to 10 gRNAs as a single transcript.

bioRxiv · May 25, 2018Bioengineering