Comprehensive double-mutant analysis of the Bacillus subtilis envelope using double-CRISPRi
Byoung‐Mo Koo, Horia Todor, Jiawei Sun, Jordi van Gestel, John S. Hawkins, Cameron C. Hearne, Amy B. Banta, Kerwyn Casey Huang, Jason M. Peters, Carol A. Gross
The paper uses double-CRISPRi to systematically analyze genetic interactions in Bacillus subtilis, identifying over 1000 interactions and revealing roles of paralogous genes in cell division.
bioRxiv · Aug 14, 2024 · PreprintGenomics