Carol A. Gross

The phenotypic landscape of the model firmicute Bacillus subtilis

This study uses CRISPRi to screen B. subtilis genes across 150 stress conditions, revealing new roles for conserved firmicute genes in protein secretion, cell wall homeostasis, and bacterial competition.

Predicting the protein interaction landscape of a mycobacterial pathogen

The paper uses AI-based protein-protein interaction predictions to characterize protein function in mycobacterial pathogens, identifying key interactions and functions for previously uncharacterized proteins.

Predicting the protein interaction landscape of a free-living bacterium with pooled-AlphaFold3

The paper predicts protein-protein interactions in Mycoplasma genitalium using a pooled-AlphaFold3 approach, improving accuracy and efficiency over traditional methods.

The phenotypic landscape of the mycobacterial cell

The paper performs a genome-wide CRISPRi screen in Mycobacterium smegmatis to uncover novel gene functions and drug targets related to the cell envelope.

Comprehensive double-mutant analysis of the Bacillus subtilis envelope using double-CRISPRi

The paper uses double-CRISPRi to systematically analyze genetic interactions in Bacillus subtilis, identifying over 1000 interactions and revealing roles of paralogous genes in cell division.

Three bacterial DedA subfamilies with distinct functions and phylogenetic distribution

The paper identifies three distinct DedA subfamilies in bacteria with different phylogenetic distributions and potential functions.

Chemical-genetic interrogation of RNA polymerase mutants reveals structure-function relationships and physiological tradeoffs

The paper uses chemical-genetic analysis of RNA polymerase mutants to uncover structure-function relationships and physiological trade-offs in Escherichia coli.

Rewiring the specificity of extra-cytoplasmic function sigma factors

The paper deciphers the DNA-specificity code of ECF σ factors to predict their regulons and reveals their varied regulatory roles in bacterial gene regulation.

Expansion and re-classification of the extracytoplasmic function (ECF) σ factor family

The paper expands and reclassifies the ECF σ factor family by analyzing NCBI data, identifying 157 phylogenetic groups and significantly increasing the number of classified sequences.

Modulated efficacy CRISPRi reveals evolutionary conservation of essential gene expression-fitness relationships in bacteria

The paper develops a CRISPRi system to modulate gene expression in bacteria and shows that essential gene expression-fitness relationships are conserved across species.

Titrating gene expression with series of systematically compromised CRISPR guide RNAs

This paper develops a method to control gene expression using CRISPR interference and deep learning, revealing gene-specific expression-phenotype relationships.