Marco Jost

Lipid Changes due to Low O-GlcNAc and the Downstream Consequences

This study investigates the effects of O-GlcNAc depletion on lipid metabolism and shows that it leads to lipid remodeling and increased sensitivity to ferroptosis.

Metabolism of bilirubin to urobilinogen by human gut bacteria occurs by a branched, multi-enzyme pathway

This study identifies BilR and BilV as enzymes involved in the branched microbial metabolism of bilirubin to urobilinogen in the human gut.

Blautia wexlerae Transforms Dietary Fatty Acids to Activate Enteroendocrine Signaling and Improve Metabolic Health in Mice and Humans

This study identifies Blautia wexlerae's role in converting dietary fats into bioactive metabolites that enhance gut hormone secretion and improve metabolic health in mice and humans.

Systematic identification and characterization of regulators of aryl hydrocarbon receptor signaling

This study identifies regulators of AHR signaling in hepatocytes using CRISPR screening, revealing novel mechanisms and cell-type-specific components for therapeutic targeting.

Vaginal lactobacilli produce anti-inflammatory beta-carboline compounds.

This study identifies beta-carboline compounds produced by vaginal lactobacilli that reduce inflammation by suppressing immune signaling pathways and demonstrate therapeutic potential in a mouse model.

Microfluidics-free single-cell genomics with templated emulsification

The paper introduces PIP-seq, a microfluidics-free method for single-cell RNA sequencing that enables high-throughput, scalable, and flexible processing of samples.

Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

This paper develops compact CRISPRi sgRNA libraries and optimal effectors to improve knockdown consistency and efficiency in mammalian cells.

Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq

The paper performs genome-scale Perturb-seq to map genotype-phenotype relationships, uncovering new gene functions and stress-specific mitochondrial regulation.

Modulated efficacy CRISPRi reveals evolutionary conservation of essential gene expression-fitness relationships in bacteria

The paper develops a CRISPRi system to modulate gene expression in bacteria and shows that essential gene expression-fitness relationships are conserved across species.

GIGYF2 and 4EHP Inhibit Translation Initiation of Defective Messenger RNAs to Assist Ribosome-Associated Quality Control

The paper identifies GIGYF2 and 4EHP as translation inhibitors that suppress re-initiation of defective mRNAs, aiding ribosome-associated quality control and preventing toxic protein accumulation.

Titrating gene expression with series of systematically compromised CRISPR guide RNAs

This paper develops a method to control gene expression using CRISPR interference and deep learning, revealing gene-specific expression-phenotype relationships.

Exploring genetic interaction manifolds constructed from rich phenotypes

The paper explores genetic interaction manifolds using rich phenotypes from Perturb-seq to classify and mechanistically understand genetic interactions.

Molecular recording of mammalian embryogenesis

The paper develops a molecular recorder to track lineage and define a mouse cell fate map using scRNA-seq, revealing transcriptional convergence of endodermal cells and estimating progenitor cell numbers.

Approaches to maximize sgRNA-barcode coupling in Perturb-seq screens

This paper identifies issues with barcode-sgRNA coupling in Perturb-seq and proposes solutions to improve accuracy in single-cell CRISPR screens.