D. Theobald

Crystal Structure of Caryolan-1-ol Synthase, a Sesquiterpene Synthase Catalyzing an Initial Anti-Markovnikov Cyclization Reaction

The paper solves the crystal structure of caryolan-1-ol synthase with and without a substrate analog, revealing structural changes that suggest a mechanism for anti-Markovnikov cyclization.

The most probable ancestral sequence reconstruction yields proteins without systematic bias in thermal stability or activity

The paper tests if resurrecting the single most probable ancestral sequence introduces bias in enzyme properties and finds no systematic bias in thermal stability or activity.

Extant Sequence Reconstruction: The accuracy of ancestral sequence reconstructions evaluated by extant sequence cross-validation

This paper evaluates ancestral sequence reconstruction accuracy using extant sequence cross-validation, finding that better models produce more biophysically accurate reconstructions despite lower sequence identity.

Bayesian machine learning analysis of single-molecule fluorescence colocalization images

The paper introduces a Bayesian machine learning method for analyzing single-molecule fluorescence colocalization images, improving accuracy by accounting for uncertainties in image data.

Bimodal evolution of Src and Abl kinase substrate specificity revealed using mammalian cell extract as substrate pool

This study reveals the bimodal evolution of Src and Abl kinase substrate specificity using mammalian cell extract as a substrate pool, showing divergent evolutionary paths leading to specific and promiscuous enzymes.

Functional and structural resilience of the active site loop in the evolution of Plasmodium lactate dehydrogenase.

The study shows PfLDH activity remains resilient to loop perturbations, suggesting alternative insertions could evolve LDH specificity as long as they contain a tryptophan in the correct position.