N. J. Krogan

Regulation of virion production by the ORF8 signal peptide across SARS-CoV-2 variants

This study shows ORF8 suppresses viral particle production by interacting with Spike and causing Golgi fragmentation, supporting evolutionary pressure to delete ORF8 in SARS-CoV-2 variants.

Protein interaction map of APOBEC3 enzyme family reveals deamination-independent role in cellular function

The paper identifies novel protein and RNA interactions of APOBEC3 enzymes, revealing deamination-independent roles in RNA biology and cancer.

Epigenetic reprogramming shapes the cellular landscape of schwannoma

This study identifies two molecular groups in schwannomas driven by epigenetic reprogramming and shows ionizing radiation can reprogram neural crest to immune-enriched schwannomas.

Cross-family small GTPase ubiquitination by the intracellular pathogen Legionella pneumophila

This study identifies how Legionella pneumophila modifies host ubiquitination during infection, revealing widespread monoubiquitination of small GTPases and the role of SidC/SdcA effectors.

A single inactivating amino acid change in the SARS-CoV-2 NSP3 Mac1 domain attenuates viral replication and pathogenesis in vivo

This study shows that mutating the SARS-CoV-2 NSP3 Mac1 domain reduces viral replication and pathogenesis in vivo.

HBV rewires liver cancer signaling by altering PP2A complexes

This study identifies how HBV alters PP2A complexes to drive liver cancer signaling, revealing potential therapeutic targets.

Structure and dynamics of the essential endogenous mycobacterial polyketide synthasePks13

This paper determines the structure and dynamics of the mycobacterial polyketide synthase Pks13 using cryoEM and complementary techniques, revealing intermediate states and substrate binding.

Deficiency in Galectin-3, -8, and -9 impairs immunity to chronic Mycobacterium tuberculosis infection but not acute infection with multiple intracellular pathogens

Impact of SARS-CoV-2 ORF6 and its variant polymorphisms on host responses and viral pathogenesis.

This study shows SARS-CoV-2 ORF6 disrupts nucleo-cytoplasmic trafficking, inhibits IFN signaling, and modulates viral protein expression, with variant polymorphisms affecting its function.

De novo protein identification in mammalian sperm using high-resolution in situ cryo-electron tomography

The paper identifies novel sperm proteins in mammals using cryo-electron tomography and AlphaFold2 models, revealing their structural roles in flagellar mechanics.

Structural insights into regulation of the PEAK3 scaffold by 14-3-3

This paper reports the cryo-EM structure of PEAK3 pseudokinase in complex with 14-3-3, revealing an asymmetric binding mechanism and identifying Protein Kinase D as a regulator.

Structure-function analysis of enterovirus protease 2A in complex with its essential host factor SETD3

The paper reports the cryo-EM structure of SETD3 interacting with enterovirus protease 2A and shows that mutations in the SET domain reduce binding and protect against infection.

A Legionella toxin mimics tRNA and glycosylates the translation machinery to trigger a ribotoxic stress response.

This paper identifies SidI, a Legionella toxin that mimics tRNA, glycosylates the translation machinery, and triggers a ribotoxic stress response leading to cell death.

Chlamydia trachomatis effector Dre1 interacts with dynactin to reposition host organelles during infection

This study identifies Dre1, a Chlamydia effector that interacts with dynactin to reposition host organelles during infection, enhancing bacterial fitness.

Type I IFN promotes pathogenic inflammatory monocyte maturation during H5N1 infection

This study shows that type I IFN promotes the maturation of inflammatory monocytes into pathogenic FasL+MCs during H5N1 infection, contributing to disease severity.

SIRT5 is a proviral factor that interacts with SARS-CoV-2 Nsp14 protein

This study identifies SIRT5 as a proviral factor that interacts with SARS-CoV-2 Nsp14, enhancing viral replication and suggesting it as a therapeutic target.

Hepatitis C Virus Infects and Perturbs Liver Stem Cells

This study identifies liver stem cells as targets of HCV infection, showing their transcriptional reprogramming and role in liver damage and cancer risk.

Evolution of enhanced innate immune evasion by the SARS-CoV-2 B.1.1.7 UK variant

This study identifies that the B.1.1.7 SARS-CoV-2 variant enhances innate immune evasion through increased Orf9b and Orf6 expression and activity.

Oncogenic PKA signaling stabilizes MYC oncoproteins via an aurora kinase A-dependent mechanism

This study identifies two PKA-driven oncogenic mechanisms, showing AURKA stabilizes MYC oncoproteins through a PIM2-mediated feedback loop, and suggests CD-532 as a potential therapeutic target.

Phospholipidosis is a shared mechanism underlying the in vitro antiviral activity of many repurposed drugs against SARS-CoV-2

Comparative Analysis of T Cell Spatial Proteomics and the Influence of HIV Expression

The paper compares machine learning methods for spatial proteome classification in HIV-expressing T cells and identifies Nef-dependent interactors.

A ciliopathy complex builds distal appendages to initiate ciliogenesis

The paper identifies the DISCO complex, essential for ciliogenesis, and shows its role in distal appendage formation and centriole length regulation, linking defects to human ciliopathies.

IL-10 driven memory T cell survival and Tfh differentiation promote HIV persistence

The paper shows IL-10 promotes HIV persistence by supporting memory T cell survival and Tfh differentiation, and inhibiting IL-10 reduces HIV reservoirs.

The CCL2 Chemokine Promotes Early Seeding of the Latent HIV Reservoir

This study shows that CCL2 chemokine promotes early HIV reservoir formation by recruiting CCR2/5+ CD4 T cells to sites of inflammation during acute infection.

Host-directed therapies against early-lineage SARS-CoV-2 retain efficacy against B.1.1.7 variant

This study evaluates the efficacy of host-directed therapies against the B.1.1.7 SARS-CoV-2 variant and finds similar antiviral activity compared to early-lineage SARS-CoV-2.

Endosomal cAMP production broadly impacts the cellular phosphoproteome

The paper shows that endosomal cAMP production leads to distinct phosphoproteomic changes compared to plasma membrane cAMP, with a strong bias toward dephosphorylation by PP2A-B56{delta}.

Zika Virus Infection Prevents Host mRNA Nuclear Export by Disrupting UPF1 Function

This study shows ZIKV infection disrupts UPF1 function, leading to nuclear mRNA accumulation and impaired neural progenitor cell function.

Functional genomic screens identify human host factors for SARS-CoV-2 and common cold coronaviruses

The paper identifies host factors and pathways common to SARS-CoV-2 and common cold coronaviruses using CRISPR screens, revealing shared and unique factors that support viral infection.

Bimodal evolution of Src and Abl kinase substrate specificity revealed using mammalian cell extract as substrate pool

This study reveals the bimodal evolution of Src and Abl kinase substrate specificity using mammalian cell extract as a substrate pool, showing divergent evolutionary paths leading to specific and promiscuous enzymes.

Cardiac Cell-Derived Matrices Impart Age-Specific Functional Properties to Human Cardiomyocytes

The paper develops a method to create stable cardiac cell-derived matrices and shows that fetal and adult matrices impart distinct functional properties to cardiomyocytes, with collagen VI in fetal matrices enhancing oxidative respiration.