Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesised proteins
Patrick Hellwig, Daniel Kautzner, Robert Heyer, Anna Dittrich, Daniel Wibberg, Tobias Busche, Anika Winkler, Udo Reichl, Dirk Benndorf
The paper develops a workflow to enrich newly synthesized proteins in microbial communities using BONCAT and click chemistry, demonstrating its application in tracing microbial activity.
ISME Communications · Jan 1, 2024Peer-reviewedMicrobiology