E. N. Senning

Genetic code expansion, click chemistry, and light-activated PI3K reveal details of membrane protein trafficking downstream of receptor tyrosine kinases

The paper uses optogenetic PI3K activation and genetic code expansion to study RTK signaling, showing PI3K alone can traffic TRPV1 and insulin receptors to the plasma membrane.

Fluorescence labeling strategies for the study of ion channel and receptor cell surface expression: A comprehensive toolkit for extracellular labeling of TRPV1.

This paper develops two orthogonal strategies to label TRPV1 on the cell surface with minimal functional perturbation, enabling tracking of its expression and mobility.

Phosphatidylinositol phosphate binding domains exhibit complex dissociation properties at the inner leaflet of plasma membrane sheets

The paper measures dissociation kinetics of PH domains from plasma membranes and finds differences in koff values between AKT-PH and PLCδ1-PH, with IP3 affecting PLCδ1-PH dissociation.

Reciprocal regulation among TRPV1 channels and phosphoinositide 3-kinase in response to nerve growth factor

The paper shows TRPV1 channels and PI3K reciprocally regulate each other in response to NGF, with TRPV1's N-terminal ARD enhancing PI3K activity.