Genetic code expansion, click chemistry, and light-activated PI3K reveal details of membrane protein trafficking downstream of receptor tyrosine kinases
D.-S. Koh, A. Stratiievska, S. Jana, S. C. Otto, T. M. Swanson, A. Nhim, S. Carlson, M. Raza, L. A. Naves, E. N. Senning, R. A. Mehl, S. E. Gordon
The paper uses optogenetic PI3K activation and genetic code expansion to study RTK signaling, showing PI3K alone can traffic TRPV1 and insulin receptors to the plasma membrane.
bioRxiv · Aug 29, 2023Physiology